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KMID : 0624620120450090521
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BMB Reports
2012 Volume.45 No. 9 p.521 ~ p.525
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Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis
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Park Ji-Hye
Lee Sang-Bae
Lee Kyung-Hoon
Ahn Jee-Yin
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Abstract
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In addition to its pivotal role in neuronal survival, PI3K/Akt signaling is integral to neuronal differentiation and neurite outgrowth. However, the exact role of Akt in neuronal differentiation is still controversial. Here, we found that nuclear expression of CA-Akt resulted in unusual rapidneurite outgrowth and overexpression of KD-Akt caused multiple dendrite growth without specific axon elongation. Moreover, microarray data revealed that the expression of FOXQ1 expression was about 10-fold higher in cells with nuclear, active Akt than in control cells. Quantitative real-time PCR analysis showed that mRNA levels were upregulated in NLS-CA-Akt cells as compared to KD or EV cells. Furthermore, our FACS analysis demonstrated that overexpression of NLS-CA-Akt accumulate cells in the G1 phase within 24 h, fitting with the rapid sprouting of neuritis. Thus, our data implied that at least in this early time frame, the overexpression of nuclear, activeAkt forced cells into neurite development through probably FOXQ1regulation.
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KEYWORD
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Akt, FOXQ1, Neurite outgrowth
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